Verifying the Heavy Metal Test Kit Efficiancy
1. Take a fresh coated test tube, remove the cap and add approximately 0.5 ml (12 drops) of Solvent to the test tube.
2. Replace the cap and shake the test tube vigorously for about 30 seconds to effect dissolution of Dithizone in the solvent. Invert the test tube and let it stand using the cap as the base for another 30 seconds. The solution should now be green and fresh and ready for use.
3. Remove the test tube cap and add 2-3mL of distilled or deionised water sample and add it to the test tube. Replace the test tube cap.
4. Shake vigorously for about 5 seconds, and then invert the test tube using the cap as the base and let it stand so that the solution can be allowed to separate.
5. Read the top layer and compare it with the chart provided with the stand. It should read Green indicating no ionic Heavy Metals.
6. Now place a piece of copper wire (with insulation removed) in the test tube and shake vigorously for about 5-10 seconds.
7. The test will now show the presence of copper. This also indicates the aggressive nature of water in leaching metals.
Urine, Saliva, Perspiration and even a blood sample may be tested for Heavy Metal using the same procedure.The Heavy Metal Test Kit can also be used to determine the environmental sources of the contamination in aqueous solutions such as tap water. Since all Heavy Metal ions are water soluble, solids like food items, porcelain dishes, dust samples from carpets, wall paints and wall paper etc. can be tested for Heavy Metals after soaking them in distilled water. In addition to being an initial analytical screening tool for Urine, Saliva, Perspiration and Blood the test is also useful for finding the causes of contamination in the patient's environment.
In 1925 Helmut Fischer of the Siemens Concern in Berlin succeeded in detecting heavy metal ions by means of a dithizone process. As a reagent, dithizone is able to indicate the presence of heavy metal ions in qualitative and quantitative terms. Dithizone, in binding the metals (binds to Cu, Zn, Cd, Hg, Pb, Mn, Co, Ni,), form coloured complexes in the interior of the molecule which are soluble in nonpolar organic solvents. The coloration of these solutions is very intense, its particular coloration determined by the atomic radius of the respective metal present in the complex. The reaction times of the heavy metal ions vary; therefore, depending on their respective concentrations, different colorations may occur from which one can, in addition to the qualitative conclusions also semi quantitative ones regarding the contaminant. (At the lower ppm level, even at the ppb level).